90 shipping. 2. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. microtiter plate reader (VERSAmax, Molecular Devices). Negative (phosphate buffered saline) (Gibco Waltham, MA, USA) and positive controls. The introduction of the Molecular Devices’ SPECTRAmax PLUS microplate spectrophotometer marks the first time that photometric measurements made in a microplate format can be automatically normalized such that the resultant values are identical to those obtained with the corresponding solutions in a standard 1 cm pathlength cuvette. VersaMax Microplate Reader (Molecular Devices cat. The VMax Microplate Reader is capable of reading up to two wavelengths at a time in the visible wavelength spectrum (400nm-750nm), with 4 filter choices included at time of purchase. Furthermore, VersaMax is reliable, which means more uptime and lower long-term cost of. Molecular Devices Versamax Tunable Microplate Reader is available from The Lab World Group. The mixture was incubate at 37 °C for 30 min, and then the reaction was stopped by the addition of 0. Tunable, filterless flexibility The VersaMax reader uses a grating monochromator to select the wavelength, so the exact wavelength needed can be selected for every assay. Microplate readers from Molecular Devices are designed to provide consistent performance over a long period of time. . MOLECULAR DEVICES VMAX KINETIC MICROPLATE READER. Although this manual has been prepared with every precaution to ensure accuracy, Molecular DevicesThe amount of extracted crystal violet was measured at 595 nm using a microplate reader (VersaMax, Molecular Devices Co. 5 mM was used to make the. , VersaMax; Molecular Devices) Incubator for cell culture. The absorbance at 570 nm was determined by VersaMax (Molecular Devices, CA) to estimate MTT-formazan production after 24, 48, and 72 hours of incubation to evaluate the proliferation rate. Youmustensurethatthemeniscusiscomfortablyabove thelightbeaminstandardcuvettesandthatthesamplechamberinamicrocuvetteis Microplate Reader Capability > UV - Vis Absorbance. The absorbance was measured at 540 nm using a hybrid microplate reader (VersaMax; Molecular Devices, Sunnyvale, CA, USA). Add to Cart. After 72-h of incubation, CellTiter 96 Aqueous One Solution was added and cells were incubated for a further 2 h. Cell viabilities were presented as the ratio of the absorbance of treated cells to the absorbance of control cells cultured with an extract of HDPE. An aliquot of the extracts dissolved in DMSO with 80 μL of phosphate buffer (pH 6. Full size table. If the computer is connected to the Internet: Select Search Automatically for updated driver software. Molecular Devices also offers a mass spectrometry-based solution for food safety testing We would like to show you a description here but the site won’t allow us. repurposing of drug molecules already approved, or in clinical trials, for other viruses. EUROPE. 9. Experimental data analysis was performed with a one-way analysis of variance (Anova with Newman-Keuls post-test) in Prism 6. Conventions Used in this Manual. For the name of the nearest authorized Molecular Devices Corporation service facility, please contact Molecular Devices at one of the following telephone numbers: (408) 747-1700 (800) 635-5577 (U. TECHNICAL NOTE. It provides both rapid and sensitive measurements of a variety of analytes across a wide range of concentrations. Tunable, filterless flexibility The VersaMax reader uses a grating monochromator to select the wavelength, so the exact wavelength needed can be selected for every assay. The variations include adjustable speed and volume, adjustable aspiration speed and time, adjustable soak times, and three modes of shaking. A solution of 10 millimolar L-DOPA (40 µL) was added to each sample and the blank. 0 nm full width half maximum. The assays were conducted in a 96-well microplate using an ELISA microplate reader (VersaMax Molecular Devices, United States) to measure absorbance at 475 nm. This user guide provides detailed instructions on how to operate, maintain, and troubleshoot the device, as well as examples of applications and. Supplier: Molecular Devices. Electrolyte leakage analysis was conducted using 8-day-old rice seedlings before and after cold stress treatments in different time points (0, 6, and 11 days) at 4°C. Wavelength Selection. The absorbance was measured at 450 nm using a microplate reader (VersaMax, Molecular Devices). Measurement of biofilm formation inhibitory activity. HUVECs were seeded into 96-well plates at a density of 30,000 cells/well and allowed to attach for 12 h. HepG2 cells were seeded into 6-well plates. 0A 2 Lines Fused, unplug before ser. Cell survival rate of each sample was calculated as a percentage relative to cells incubated in D-MEM. Statistical analysisThe absorbance was measured using a microplate reader (VERSAmax; Molecular Devices, San Jose, CA, USA) at an excitation wavelength of 530–560 nm. Biosystems CytoFluor 4000 Multi Well Plate Reader $ 1,995. Monday to Friday 8am - 5pm GMT. The mouse hippocampal neuronal cell line HT22 is frequently used as an in vitro model to investigate the role of hippocampal cholinergic neurons in cognitive functions. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate Spectrophotometer. The absorbance of the mixture was measured with a microplate reader (VERSAmax™, Molecular device, Sunnyvale, USA) at wavelength of 498 nm until absorbance was saturated. 0200-6117. Manufacturer: Molecular Devices Model: VERSA MAX Turnable Microplate reader Further Information Descriptions, data, dimensions and quantities are based on the subjective beliefs of the auctioneer and are created to the best of our knowledge and belief. ¨We would like to show you a description here but the site won’t allow us. SoftMax® Pro, the industry-leading microplate reader software, is included at no additional charge. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. In this study, we have prepared thermoreversible PG containing emodin with high emodin solubility and the gelation temperature suited to physiological use. the plate reader (model VERSAmax, Molecular Devices, USA) at 515 nm (A515). for cell lysis via visual inspection and optical microscopy (VersaMax, Molecular Devices, Sunnyvale, CA, USA) observation and checked by measuring the absorbance at OD680. Molecular Devices E Max Precision Microplate Reader. Manufacturer: Molecular Devices; Includes PC Workstation w/ Softmax Pro Software Wavelength Range (nm): 340–850 Wavelength Accuracy: < ±0. Cyanobacterial strains that did not lyse until the 12th day were defined as insusceptible. , Sunnyvale, CA, USA). VersaMax ELISA Microplate Reader. While the cell covers about. Wavelength Accuracy. Coat plate with 100 μL each of appropriately diluted standards (Table 1),. For pinocytosis, cells in wells were washed in PBS and 5 mg/ml of HPTS dye (in PBS) added and incubated with gentle rocking at 30 °C for 120 min. p-nitrophenol (Sigma) with diluted concentrations ranging from 0 to 0. Molecular Devices SoftMax Pro 5. com ©2010 molecular devices, inc. Optical Densitiy (OD) values were processed with a data reduction software (SoftMax Pro, Molecular Devices) using a 4-parameter logistic fitting algorithm to the standard curve, allowing the determination of antibody. 00 USD. The activity was measured colorimetrically at 450 nm using a microplate reader (Versamax, Molecular Devices, Sunnyvale, CA, USA). Whether its cell line development, 3D biology, drug screening—or a combination of the three—our technology maximizes throughput and reproducibility to give. FRAP solutions were prepared as described previously (Benzie and Strain, 1996, Benzie and Strain, 1999). Then, the cultured cells were treated with different. It was verified that absorbance values were proportional to the amount of a bound peroxidase-labeled antibody. Analytical Technologies Group specializes in the maintenance and repair of Molecular Devices microplate readers, including the SpectraMax® and VersaMax™ absorbance readers. 0A Sunnyvale, CA Made in USAVersaMax price. Microplate Reader Capability > UV - Vis Absorbance. The SpectraTest® ABS1 Absorbance Validation. A standard curve, provided by the manufacturer, was generated and the sample concentrations determined by 4-parameter logistic curve. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. absorbance readers. MiR-21. StakMax Microplate Handling System. Supplier: Molecular Devices. The mouse hippocampal neuronal cell line HT22 is frequently used as an in vitro model to investigate the role of hippocampal cholinergic neurons in cognitive functions. Molecular Devices SpectraMax Plus 384 Microplate Reader *Parts Only* Tecan Sunrise Microplate Reader. The SpectraMax® QuickDrop™ UV-Vis Spectrophotometer quantifies very small amounts of DNA, RNA, oligos, and proteins. Concluding Remarks. For the name of the nearest authorized Molecular Devices Corporation service facility, please contact Molecular Devices at one of the following telephone numbers: (408) 747. 8). on the similarities in performance between four single-mode absorbance plate readers: SpectraMax® ABS, SpectraMax® ABS Plus, SpectraMax® 190* and VersaMax™* Microplate Readers. Am J Respir Crit Care Med 1998; 157: 351–370. Access this by going to. 00 Add to cart Molecular Devices Microplate Reader Spectramax 384 340PC $ 6,295. JEG-3 cells were transfected with myc-tagged NDRG1 wild type or deletion mutants at the N- or C- terminal as depicted in Figure 5A. Afterward, HUVECs were incubated with EPC-EVs, TFEB-EVs, or PBS for 24 h or 48 h. Expand the Port (COM & LPT) Right click on the USB Serial Port (COMx) and select Properties. Regular price $ 0. Spectranet customer portalThe absorbance was recorded at 532 nm using a microplate reader (VERSAmax, Molecular Devices, Sunnyvale, USA). , Sunnyvale, USA). 00. The product formed was then transferred to 96 well plate and the absorbance was measured at 540 nm using microplate reader (VersaMax™, Molecular Devices, CA, USA). It features a dual-monochromator system, a large dynamic range, and a high-throughput capability. 0A Sunnyvale, CA Made in USA The Molecular Devices VersaMax ELISA Microplate Reader enables precise and accurate detection at wavelengths in the visible spectrum without the use of filters, providing consequence-free assay flexibility. S. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard. The optical densities (ODs) of the reactants were measured at 450 nm using a microplate spectrophotometer (Versamax, Molecular Devices, CA, USA). Fabrication of Microfiber-Based Strain SensorsWe would like to show you a description here but the site won’t allow us. Molecular Devices versamax Operator's Manual (47 pages) Tunable Microplate Reader. from Molecular Devices Corporation. ImmunohistochemistryAfter 1 h incubation at 37 °C, the color intensity was measured at 562 nm by a microplate reader (model VERSAmax; Molecular Devices, San Jose, CA, USA). OptiMax (Versamax non EXT) B0: Screenstation: all: Skanstacker: all: Skanwasher 300: all: Skanwasher 400: all: SpectraMax 190: N0:. 0. Select Add or Remove Programs system settings. The SpectraTest® ABS1 Absorbance. Innovative and flexible design features enable accelerated sample preparation time and increased laboratory productivity of DNA, RNA, and protein samples. In general terms, plasma was added to a 96-well plate containing the targeted antisera, after rigorous washing steps and conjugation with a secondary detection antisera, and the resultant colorimetric reaction was read by scanning spectrophotometry (VersaMax, Molecular Devices, Sunnyvale, CA). The viability of the treated group was expressed as a percentage of non-treated control group, which was assumed to be 100%. Whether its cell line development, 3D biology, drug screening—or a combination of the three—our technology maximizes throughput and reproducibility to give. +86-10-6410-8669 fax +86-10-6410-8601 Molecular Devices Shanghai Tel. Used. Tested w/warranty. SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers. After treatment, cells were collected, lysed with the lysis buffer. Glossary of Terms. With a wavelength range from 340 to 850 nm, it is the equivalent to having 510 free filters. 00 USD. Windows Home Editions are not supported. The cells were seeded on the bottom of 24-well plates with sterile glass coverslips. Locate the User Guide on your SoftMax Pro computer for version 6 and version 7. P. The samples were incubated with FBS, and the optical density of particles was determined with a microplate reader (VERSAmax, Molecular Devices, Sunnyvale, CA) by measuring the absorbance at 560 nm for 2 h. The Molecular Devices VersaMax Microplate Reader is a high-performance laboratory instrument designed for fast and accurate measurements of absorbance, fluorescence, and luminescence from 6 to 384-well microplates. Also see: SoftMax® Pro Microplate Software User Manual Download Page. 5°C @ 37 °C W. It covers everything from installation, configuration, data acquisition, data analysis, curve fitting, to reporting and troubleshooting. We would like to show you a description here but the site won’t allow us. treatments were then examined atλ=570 nm using VersaMax eliSa reader (Molecular devices, llc. SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. The conversion of L-DOPA to dopachrome was allowed to proceed for 40 min at 30 °C in a microplate reader (Versamax; Molecular Devices). We would like to show you a description here but the site won’t allow us. The BMDM cells were plated into a 6-well culture plate at a density of 2. Cells were lysed in NETN buffer (100 mM NaCl, 20 mM Tris pH 8. The VersaMax™ Microplate Reader from Molecular Devices bridges the gap between the affordability of filter-based readers and the flexibility of monochromator-based systems. Read-out was performed within 30 minutes with a microtiter plate reader at 450 nm (Versamax, Molecular Devices). The VersaMax™ Microplate Reader from Molecular Devices bridges the gap between the affordability of filter-based readers and the flexibility of monochromator-based systems. reader (VersaMax, Molecular devices, San Jose, CA, USA). Select Update Driver. The limit of detection for each assay was 156. Ann Arbor, MI, USA). Home - University of Victoriaimported directly into Excel (Microsoft, Redmond, WA) from the ELISA microplate reader (VersaMax; Molecular Devices, Sunnyvale, CA, USA). All cell lines were cultivated for 24 h in 96-well cell culture plates with an initial concentration of 1×10 5 cells/mL. 5) containing 0. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. The VersaMax™ Microplate Reader from Molecular Devices bridges the gap between the affordability of filter-based readers and the flexibility of monochromator-based systems. and California VERSAMAX Tunable Microplate Reader 100-240V - 4A 50-60 HZ T4. The sections were stained with hematoxylin and eosin (H&E) and. And the VERSAmax reader is robotics-compatible. MDC instruments including: SpectraMax®, VersaMax. The emodin thermoreversible PG was. To date, over 219 million cases and 4. The SpectraMax® absorbance spectrophotometers and plate readers provide the versatility and convenience for a wide range of assays such as ELISAs, nucleic acid and protein quantitation, and microbial growth. versamax, vmax, emax, uvmax, flexstation, softmax pro 5 gxp software, spectratest, molecular devices automation partners, gemini, lmax, assay protocols, data display, data analysis, assay. Finally, a 5% glucose solution was added to the suspension of CPN and FCPN as a freeze-dried protective agent. Battery Backup Uninterruptible Power Supply (UPS) And Power Conditioner For MDS Sciex Molecular Devices VERSAmax Microplate Reader Quote Request. Manufacturer: Molecular Devices The Molecular Devices VersaMax Tunable Microplate Reader for 96-well format uses a grating monochromator to select the wavelength, so the exact wavelength needed can be selected for every assay. All parts of the SpectraTest ABS1 Validation Plate are warranted for a period of ninety (90) days from the original date of. The Molecular Devices SpectraMax XPS has an optical design that is the only multi-channel system that truly mimics a dual-beam spectrophotometer. 635. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. Molecular Devices Corporation Instrument Warranty Molecular Devices Corporation warrants this product against defects in material or workmanship as follows: 1) All parts of the VERSAmax Tunable Microplate Reader are warranted for a period of one (1) year from the original date of delivery. FAST AND ACCURATE METERTECH Microplate Reader $3,650. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes. The wells were visualized using o-phenylenediamine (OPD), and the absorbance was read with a multiwall spectrophotometer (VERSAmax, Molecular Devices, Sunnyvale, CA, USA) at 490 nm. from Molecular Devices Corporation. Cell viability was analyzed using a cell counting kit assay (Dojindo). ®ABS2, FL1, and LM1 Validation Plates sent to Molecular Devices for recertification at our ISO 17025 accredited lab are disassembled, cleaned, calibrated, an then returned with a new certificate of calibration. The cellular viability. MOLECULAR DEVICES VersaMax Reader: Model #: VersaMax: Serial #: N/A: Year Built: N/A: Location: California: Price: $3,800. All samples were measured in triplicate. , Sunnyvale, CA, USA). We would like to show you a description here but the site won’t allow us. S. This document is provided to customers who have purchased Molecular Devices, Inc. The PathCheck Sensor is a revolutionary new awardwinning1 technology in SpectraMax® Instruments for measuring the fluid level in every well of a microplate 2–5. ® US HEADQUARTERS. The plate was read at 570 nm using an ELISA reader (VERSAMax, Molecular Devices, VWR, Philadelphia, PA, USA). Used. The detection limit for rivaroxaban in this assay was 10 ng/mL. Cell viability was represented by percentage values compared to a. Statistical analysis Graphs and statistics were prepared in GraphPad Prism, version 7. This manual is subject to change without notice. This robust model allows precise wavelength detection in. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. Battery Backup Uninterruptible Power Supply (UPS) And Power Conditioner For MDS Sciex Molecular Devices VERSAmax Microplate Reader Quote Request. Molecular Devices SoftMax Pro 5. The plates were immediately measured at 405 nm for 6 min using a microtiter plate reader (VersaMax; Molecular Devices, Sunnyvale). 9 in. The RT-qPCR analysis was performed on a 7500 Fast Real-Time PCR system (Applied Biosystems, USA). The absorbance was measured at 570 nm and. Molecular Devices GMBH Tel. 5 mM. The absorbance was then determined by an enzyme-linked immunosorbent assay (ELISA) reader (VERSAmax; Molecular Devices, Sunnyvale, CA) at a wavelength of 405 nm. 2. S. Molecular Devices Spectramax Plate Reader. SoftMax® Pro Software is the leading solution for microplate reader control and data analysis, where our exclusive INSIDER members help to ensure it remains the most innovative research tool available. It features a modular design that allows users to add or change detection cartridges according to their needs. The assay was performed in 96-well microtiter plates in duplicate, and the absorbance was read at 405 nm with 620 nm as the reference wavelength using a microplate reader (VersaMax, Molecular Devices, Boston, Massachusetts). MDC instruments including: SpectraMax®, VersaMax. Monitor reader performance on demand to ensure data quality is not compromised between PM/OQ. Find regional support. 3 Methods 1. The absorbance value (OD) was measured at 492 nm with a microplate reader (VERSAmax, Molecular Devices, Sunnyvale, CA). The cells were treated with 15, 25, 30, and 45 nm-particles in order to compare the cytotoxicities of different sized nanoparticles on the treated cells. Tunable, filterless flexibility The VersaMax reader uses a grating monochromator to select the wavelength, so the exact wavelength needed can be selected for every assay. 1700 +1 800. Cells were treated with 150 μl of DMSO and absorbance was quantified in 540 nm using the microplate spectrophotometer system (VersaMax, Molecular Devices, Sunnyvale, CA, USA). Molecular Devices FMax Thermo Fluoroskan Ascent Fluorescence Microplate Reader W WARRANTY $3,450. Spectranet customer portalSpectraMax and VersaMax . In the Apps and Features window, locate and uninstall SoftMax Pro. Bimolecular fluorescence complementation (BiFC) systemMolecular Devices Versamax Tunable Microplate Reader is available from The Lab World Group. Cell proliferation. After addition of stop solution, the optical density of samples were read at wavelengths of 450 and 570 nm (Versamax, Molecular Devices Co. Our multi-mode plate readers provide great flexibility and include absorbance, fluorescence and luminescence with configurable options for fluorescence polarization (FP), time. Table 1. SpectraMax®, VersaMax, VMax. The SpectraMax Plus 384 Microplate Spectrophotometer is a versatile and reliable instrument that can measure absorbance in the UV and visible range. +81-6-6399-8211 fax +81-6-6399-8212 Nihon Molecular Devices Tokyo Tel. BMG Labtech - THERMOstar. on the similarities in performance between four single-mode absorbance plate readers: SpectraMax® ABS, SpectraMax® ABS Plus, SpectraMax® 190* and VersaMax™* Microplate Readers. VersaMax Absorbance Microplate Reader memungkinkan pemilihan hingga dua panjang gelombang sekaligus untuk deteksi absorbansi dalam. Molecular Devices Corporation reserves the right to change its products and services at any time to incorporate technological developments. Bovine serum albumin was used as a reference. 635. 747. The concentrations of anti-RSV G protein IgG antibodies in the samples were. However, whether these cells exhibit the serotonergic neuronal phenotype observed in mature hippocampal neurons. 2 or higher, standard edition only, no GxP) VersaMax™ Microplate Reader (Serial numbers begining with BNxxxxx and BNRxxxxx are supported) SpectraMax® 190 Absorbance Microplate Reader (Serial. Approximately. Manual for Molecular Devices versamax Laboratory Equipment (47 pages) Specifications: 1379/1379020-versamax. With a wavelength. Built around a grating monochromator, the VersaMax™ Absorbance Microplate Reader allows for selection of up to two wavelengths at a time for absorbance detection in the visible wavelength range (340–850nm). Cell proliferation assay MDA-MB-231 cells were seeded at a density. In this study, we have prepared thermoreversible PG containing emodin with high emodin solubility and the gelation temperature suited to physiological use. Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. The emodin thermoreversible PG was. Table of Contents. Multichannel optics. New and used Molecular Devices VersaMax's for sale on LabX. For more advanced automation needs, Molecular Devices interacts with all of the major lab automation providers, and is one of their leading choices. In brief, mature 3T3-L1 cells were washed with DPBS, fixed with 10% formalin for 1 h at room temperature, washed once with 60%. 4 (Molecular Devices cat. Tunable, filterless flexibility The VersaMax reader uses a grating monochromator to select the wavelength, so the exact wavelength needed can be selected for every assay. 10. Page 6 SpectraMax Plus384, 190, 340PC384, and VersaMax. Spectrophotometers. SpectraMax and VersaMax Plate Readers Operator s Manual -- 0112-0126 Rev A Molecular De. 1. The VersaMax™ Microplate Reader from Molecular Devices bridges the gap between the affordability of filter-based readers and the flexibility offered by monochromator-based systems. VERSAMAX. Cet appareil ISM est confomre à la norme NMB-001 du Canada. VersaMax Absorbance Microplate Reader memungkinkan pemilihan hingga dua panjang gelombang sekaligus untuk deteksi absorbansi dalam. ChE activity was calculated as the rate of change of absorbance per minute during the linear portion of the reaction. 1. All animal experiments were performed in compliance with the relevant laws and. SpectraTest ABS1 Absorbance Validation Plate. Monitor reader performance on demand to ensure data quality is not compromised between PM/OQ. A. The VersaMax™ Microplate Reader from Molecular Devices bridges the gap between the affordability of filter-based readers and the flexibility of monochromator-based systems. 6 nm, respectively, by ELISA microplate reader (VERSAmax, Molecular Devices, USA). Primer design. VERSA MAX-LUNABLE, MOLECULAR DEVICES, MICROPLATE R. s. Monochromator, tunable in 1-nm increments. Spectranet customer portal. Ensure data accuracy of your reader. The absorbance was read at 570 nm using a spectrophotometer (VersaMax, Molecular Devices, San Jose, CA, USA). A. Absorbance was determined at 490 nm on a microplate reader (VersaMax; Molecular Devices, Sunnyvale, CA, USA). No accessories are needed and SoftMax® Pro Software. This user guide provides detailed instructions, troubleshooting tips and technical specifications for these instruments. We would like to show you a description here but the site won’t allow us. Monday to Friday 7am - 5pm Pacific Time. +49-89-9620-2340 Fax +49-89-9620-2345 Japan Nihon Molecular Devices Tel. Versamax Microplate Reader dibangun di sekitar monokromator kisi. 00 Sale price $ 0. The Molecular Devices VersaMax Microplate Reader is a high-performance laboratory instrument designed for fast and accurate measurements of absorbance, fluorescence, and luminescence from 6 to 384-well microplates. 0 OD. S. ~ Bio-Tek ELx808 Microplate Reader $2,795. Reboot the computer. The VersaMax Absorbance Microplate Reader allows for selection of. Quantitative real-time polymerase chain reaction For quantitative real-time polymerase chain reaction (qRT-PCR) analysis, total cellular RNA was extracted from ten zebrafish larvae. Intracellular triglyceride assay. 8. Cell viability was expressed as percent survival of untreated cells. Tunable, filterless flexibility The VersaMax reader uses a grating monochromator to select the wavelength, so the exact wavelength needed can be selected for every assay. SpectraMax® i3 Multi-Mode Detection Platform. Nude mice were obtained from Orient Bio Inc. Tunable, filterless flexibility The VersaMax reader uses a grating monochromator to select the wavelength, so the exact wavelength needed can be selected for every assay. The activity was measured at 40 °C following o-nitrophenol (ONP) release at 405 nm using a microplate reader (Versamax, Molecular Devices, San Jose, CA, USA). 00. SoftMax Pro. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. Wavelength Range. Cell viability was assessed using the WST-1 assay. The signal produced was proportional to the amount of analyte present and interpolated from the calibration curve presented on each plate. Although this manual has been prepared with every precaution to ensure accuracy, Molecular Devices SpectraMax and VersaMax Plate Readers OperatorÕs Manual Ñ 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. 2. High-Throughput Screening. The Molecular Devices VersaMax Tunable Microplate Reader for 96-well format uses a grating monochromator to select the wavelength, so the exact wavelength needed can be selected for every assay. , St. A. S. The average OD 490 of 12 blank wells (Line 18) was then subtracted from the raw OD 490 obtained for each sample well. We would like to show you a description here but the site won’t allow us. Get pricing and purchase lab equipment and. 0 out of 5 $ 2,999. Briefly, at 3 h before each of the desired time points, 10 µl of the MTS reagent. To update the driver. ChE activity was normalized by protein concentration as determined by a Coomassie. 사용이 편리하고 견고한 생명과학 연구, 신약 개발, 개발, Bioassay용 assay. Microplate Readers Molecular Devices Spectramax iD3 Microplate Reader. Products : General, Microplate Readers. To update the driver. All the experiments were conducted at. Turn the reader on and secure the USB cable. The reaction was started by adding 10 µL of the enzyme solution (properly diluted) to 190 µL of 15 mM ONPG in 0. 4. Cell viability was represented by percentage values compared to a control and then used to calculate the number of viable cells. The collaborative space brings customers and researchers into the lab to test automated workflows for organoid culturing and screening, with guidance from in-house. #0120-1293A SpectraMax, SoftMax Pro, Vmax and Emax are registered trademarks and VersaMax, Lmax,Although this manual has been prepared with every precaution to ensure accuracy, Molecular Devices Corporation assumes no liability for any errors or omissions, nor for any damages resulting from the application or use of this information. A microplate reader (VersaMax, Molecular Devices, USA) was used to detect the OD value at 450 nm. Molecular devices The VersaMax Absorbance Microplate Reader costs between $5,500 and $6,600. The preparation of cytoplasmic and nuclear proteins was performed by using NE-PER Nuclear and Cytoplasmic Extraction Reagents (Invitrogen, USA) according to. (“Molecular Devices”) equipment, software, reagents, and consumables to use in the operation of such Molecular Devices equipment, software, reagents, and. The VersaMax measures thMolecular Devices memberdayakan para ilmuwan untuk memajukan diagnosis dini dan terapi yang lebih aman untuk pasien. Learn how to operate the VersaMax, SpectraMax 340PC384 and SpectraMax 190 Plus384 microplate readers, which offer fast and accurate absorbance measurements for a variety of assays. Model: VERSAmax Tunable Microplate Reader. 17. Versamax Microplate Reader dibangun di sekitar monokromator kisi. 00: Description. Myeloperoxidase AssayThe SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. Finally, 0. 003 OD ±1. They were then pre-treated with indicated concentration of drugs for 1 h prior to being treated or not with 5-HT or serum. Absorbance was read on a spectrophotometer (VersaMax, Molecular Devices) with SoftMax Pro GxP v5 software. Download PDF versions of the SpectraMax Microplate Reader User Guides. Technical Specifications. 5×10 3 cells were seeded in 96-well plates, treated with indicated inhibitors for 48 or 72 hours, analyzed 16 hours after BrdU addition, and absorbance measured at 450nm using a spectrophotometer plate reader (VersaMax, Molecular. By measuring optical pathlength, it eliminates the last major distinction between microplate readers and spectrophotometers. SoftMax® Pro Software is the leading solution for microplate reader control and data analysis, where our exclusive INSIDER members help to ensure it remains the most innovative research tool available. For more information, please visit us at 160 µL of sample solutions/positive control (ascorbic acid) were mixed with 40 µL of DPPH solution and incubated at room temperature for 30 min. Molecular Devices VersaMax Absorbance (UV/Vis) Elisa Microplate Reader For Sale for Plate Reader is complete with computer, SoftMa. ® Standalone instrument with touchscreen and built-in sample ports for small volumes and cuvettes. The number of cells that migrated to the bottom was determined by measuring the optical density at 590 nm, using a microplate reader (VersaMax; Molecular Devices). For the name of the nearest authorized Molecular Devices Corporation service facility, please contact Molecular Devices at one of the following telephone numbers: (408) 747-1700 (800) 635-5577 (U. . The VMax Microplate Reader is capable of reading up to two wavelengths at a time in the visible wavelength spectrum (400nm-750nm), with 4 filter choices included at time of purchase. The colorimetric reaction was read using an automated ELISA microplate reader (Versamax, Molecular Devices, USA) at 492 nm. The VersaMax™ Microplate Reader from Molecular Devices bridges the gap between the affordability of filter-based readers and the flexibility of monochromator-based systems.